RNA
Spacer1

Part:BBa_K4654009:Design

Designed by: Ronja Friedhoff, Felix Jarecki   Group: iGEM23_TU-Braunschweig   (2023-10-08)


Spacer Sequence 1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

An important insight into the design of the construct came from the expertise of Prof. Dr. Mario Moerl, who emphasized the importance of maintaining the natural environment of the riboswitch (BBa_K4654011). This is crucial because it influences the folding and functionality of the riboswitch. We therefore made the decision to retain the spacer sequence between the promoter and the riboswitch.

Source

The sequence we are using was created by White et. al. (2022) by creating a consenus sequence from different sequences of the riboswtich family1.

References

1 White, N., Sadeeshkumar, H., Sun, A. et al. Lithium-sensing riboswitch classes regulate expression of bacterial cation transporter genes. Sci Rep 12, 19145 (2022). https://doi.org/10.1038/s41598-022-20695-6